Queensland Agricultural SeedsQueensland Agricultural Seeds

Inoculant Application (2 of 2)

Methods of Peat Inoculants Application

A) Calculate the quantities of materials needed for different legumes. The quantities required will vary with the size of the seed.

B) Mix the peat culture with pre-prepared adhesive making sure the solution has been cooled first.

C) Pour this mixture over the seed and stir until all the seeds are wet.

D) Add immediately the required amount of finely ground limestone and mix until the seeds are evenly coated and well pelleted. Cease mixing as soon as the pellet has been formed.

The adhesive used in pelleting provides some protection for the bacteria from the harmful effects of high temperatures and the drying associated with storage and dry sowing. However, it is best to inoculate seed as close as possible to sowing. Ideally, it should be sown into moist soil immediately.

Inoculation Chart

Australian inoculation Group Chart 2010BioStacked®Nodulaid ™ PeatNodulator™ Granules
Group TypeUsed for inoculating the following legumesEach BioStacked® kit treats 1000kg of coarse grain seedMaximum weight kgs of seed to be treated 
Standard PackLarge Pack
ALLucerne, Strand and Disc Medic 25125ü
AMAnnual Medics except Strand and Disc 50  
BWhite, Red, Strawberry, Alsike, Suckling, Berseem, Cluster Clover 25125 
CAll Subterranean Clovers, Crimson, Rose, Cupped and Helmet Clover 50250ü
 Arrowleaf, Balansa, Gland, Purple Clover, Persian (Shaftal) Clover (cv Maral) 25125ü
E*Pea, Field Pea, Vetch, Tare, Narbon Beanü 500ü
F*Faba Bean, Tick or Broad Beamü 500ü
 Lentilü 250 
G*Lupinü 500ü
 Yellow, Pink, Hybrid, Slender Serradella (seeds and pods)ü 250ü
 Soybean (NITRI-LIFE™ Liquid Inoculant 3L)ü 1000 
I*Cowpea (cv. Poona Pea, Caloona, Banjo), Mung Bean 100 ü
J*Pigeon Pea, Lablab 100 ü
 Macrotyloma axillare (cv. Archer) 50  
N*Chickpeaü 500ü
P*Peanut (FP Peat Culture) 100 ü
Biserrulacv. Casbah  50ü

* These inoculants contain Celstik™ (a built in adhesive and stabiliser)

Important Notes:

  1. Outdated BIOSTACKED®, Nodulaid ™, Nodulator™ and NITRI-LIFE™ must NOT be sold.
  2. Store BIOSTACKED®, Nodulaid ™, NITRI-LIFE™ inoculants in refrigerator or cool place ideally 4°C - 8°C (do not freeze)
  3. Store Nodulator™ in a cool, dry place
  4. Also available - Seedstik seed coating adhesive

Peat Inoculant information supplied by Becker Underwood Pty. Limited

Methods of Freeze Dried Inoculant Application

Directions for preparation of legume Inoculant solution:
EasyRhiz® is supplied in two parts which are combined before use:

  1. A glass vial of freeze dried Rhizobium bacteria for inoculating legume seed
  2. A pack of EasyRhiz® protecting agent.

This product is suitable for both coating seed prior to sowing and liquid injection during sowing. The maximum amount of seed that can be treated is indicated on the vial label. Use one pack of EasyRhiz Protecting Agent per vial.

Inoculation solution preparation
Remove cap and rubber bung from the glass vial, add clean cool water, replace bung and shake to dissolve powder. All powder must dissolve. It is preferable to use rain water with a pH between 6.5 and 7.5.

For Seed Coating:

  1. Add this pack of powder to sufficient water to treat the weight of seed indicated on the vial. As a guide 50kgs of small seed size requires 750mls water, 250kgs medium size seed requires 1.5 litres water and 500kgs large size seed requires 3 litres water. Adjust water rates as required. Using less water will shorten drying time but may reduce seed coverage.
  2. Mix in the vial of Inoculant solution prepared earlier. Rinse the vial.
  3. Coat this solution evenly over the seed to be treated.

Treated seed should be planted within 5 hours of inoculation.

For Liquid Injection:

  1. Add 2 litres of cool clean water to a clean bucket, add EasyRhiz® Protecting Agent, then add the vial of inoculant solution prepared earlier. Rinse the vial.
  2. Add this solution to the spray tank of a liquid injection system on a crop seeder or combine, at 50 to 200 litres of clean water per hectare. The liquid injection system delivers the solution into the planting furrow along with the seed during the planting process. It is important that the inoculant solution contacts the seed as it is sown.


Store the vial between 4°C and 10°C out of direct sunlight. Apply only to the specific legume seed indicated on the label of the vial.


  • Do not prepare inoculant in containers that have previously contained pesticides.
  • Do not use if sowing into soil with poor soil moisture (dry sowing)
  • Do not use if sowing into soil that is over 30°C. Do not apply to seed that is coated with toxic chemicals.
  • Note: Increased application rates improve results. Double the concentration of inoculant (ie use 2 vials instead of one) if the land has never grown this type of legume crop previously (keep the application of water standard)


As the manner of use of this product is beyond the company's control, no warranty is given other than those warranties implied by the Trade Practices Act 1974 and in any event the liability of the company is limited to replacement of this product or the payment of the cost of doing so. EasyRhiz® Freeze Dried Inoculant

EasyRhiz® Freeze Dried Inoculant

Inoculant GroupHost Plant - Common NameEasyRhiz™EasyRhiz™ Protectant
 Pack SizeVialFoil
StrainSeed treated per packKg(Powder)
AL, “Lucerne” (RR1128) Lucerne, Strand medic, Disc medic100100g
AM, “Medic” (WSM1115)Barrel medic, Burr medic, Snail medic, Sphere medic, Gama medic, Murex medic200250g
B, “White clover” (TA1)White clover, Red clover, Strawberry clover, Alsike clover, Berseem (Egyptian) clover, Cluster or Ball clover, Suckling clover100100g
C, “Sub clover”Crimson clover, Cupped clover, Helmet clover, Purple clover, Rose clover, Sub clover200250g
 Arrowleaf clover, Balansa clover, Gland clover, Persian (Shaftal)100250g
E, “Pea” (SU 303)Field pea, Grass pea, Common vetch or Tare, Bitter vetch, Lathyrus, Purple vetch, Pea, Woolly pod vetch500250g
F, “Faba” (WSM1455)Faba, Tick or Broad bean500250g
G,“Lupin” (WU425)All lupin500250g
H, “Soy” (CB1809)Soybean500250g
I, “Mung Bean” (CB1015)Cowpea, Mung bean, Moth bean, Dune bean, Rice bean, Snake bean, Creeping vigna500250g
J, “Lab Lab” (CB1024)Dolichos lablab, Pigeon pea, Hyacinth bean500250g
 Perennial horse gram (Axillaris)250250g
M, “Siratro” (CB756)Butterfly pea, Atro, Tropical kudzu, Puero200250g
 Glycine, Siratro, Jack bean, Calopo , Gambia pea, Phasey bean, Velvet bean, Banana bean, Wing bean or Goa , Wynn Cassia, Kudzu100250g
N, “Chickpea” (CC1192)All Chickpea500250g
P, “Peanut” (NC92)Peanut or Groundnut500250g
S, “Serradella” (WSM471)All Serradella200250g
Special Inoculants
5G1BAdzuki bean200250g
SU343Birdsfoot trefoil (Lotus corniculatus)25100g
CB1717Burgundy bean100100g
CC283bCaucasian ( Kura ) clover50100g
CB1923Centro, Centurion200250g
CC511French or Common bean, Navy, Kidney, Dry, Lima beans250250g
CB3035Guar or Cluster bean250250g
CB2312Jointvetch, Aeschynomene100100g
CB782Kenya white clover (Trifolium semipilosum)50100g
CC829 (Lotus)Lotus, Lotus pedunculatus25100g
CIAT3101Pinto peanut250250g
CB1650Stylo- Caribbean stylo (Stylosanthes hamata)50100g
CB3481Stylo- Caatinga stylo (Stylosanthes seabrana)50100g
CB82Stylo- All other Stylo (Fine stem, Shrubby, Townsville)50100g
WSM 1592Sulla100100g
CC1502Tree Lucerne or Tagasaste25100g

EasyRhiz™ is a soluble powder of pure culture supplied in a glass vial and comes with EasyRhiz™ protecting agent.

All Rhizobium strains used are approved by the NSW Department of Primary Industries, 2009

Store all cultures in a cool place

Freeze dried Inoculant information supplied by New-Edge Microbials Pty Ltd

Queensland Agricultural Seeds

Queensland Agricultural Seeds
Email: pastures@qaseeds.com.au   Phone: +61 7 4630 1000   Fax: +61 7 4630 1005
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